SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. ® reader to the computer. D 47 7. Example: [Drawer]. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Titration of human serum and plasma (heparin) within the working range of the assay. Fastenal is the largest fastener distributor in North America. 5 mg mL −1 with 0. Molecular Devices SpectraMax® series microplate readers. 3. Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. Description 1. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. SpectraMax® M5e. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. Data analysis methods. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Background-subtracted data values (mean +/- SD) are graphed. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. Each well was scanned at nine evenly distributed points and averaged automatically with the software. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Briefly, 4T1 cells were seeded (5000 cells/well) in a 24-well plate. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Where can I find UV/Vis Plastic Disposable Cuvettes? Some Plastic Disposable UV (300 nm +) and VIS Cuvette sources: NOTE: Some disposables with channel sides (as opposed to full. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Fluorescence Microplate Reader Comparison Chart. Category 2 and 3 microplate readers use gradient heating as well. The DQ Green BSA probe. The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. siRNA-mediated knockdown of KLF2 in RAW 264. All associated StakMax® stackers. $1,250. Dissolve FITC-dextran in PBS at a. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. /600 em. The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. All of the experiments were performed in triplicate, and the results were represented as mean. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. Description 1. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Note: In this user guide, all references to SpectraMax Multi-Mode. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Roelli1, Amandine Stoss1, Rebecca A. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. spr SpectraMax iD3 SpectraTest ABS2 v1. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 5. The available phosphorus in the soil was leached by double-acid extraction (0. 1 & 7. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. e. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. The Insulin standard curve was prepared as described in Section 10. Highly selective for dsDNA over ssDNA or RNA. /525 em. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. 7% of the total test aerosol mass) was not included in the collection analysis. 315 can't find zero order. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. 4) and cells were stained with 150 nM TSQ for 1 min. Results and Discussion Small-molecule agonists have been identified for TLR7 [ 41 , 42 , 47 , 48 , 60 – 67 ], TLR8 [ 49 , 51 – 54 , 68 ], TLR4 [ 69 – 72 ] and TLR2 [ 73 , 74 ]. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. We therefore gathered salient. Results were expressed as the mean percentage of CV compared with NC group. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. † Bottom mounting for SpectraMax Paradigm Readers only. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. spr SpectraMax iD5 SpectraTest ABS2 v1. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. S. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. 4. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. > spectramax m4 microplate reader. ZERO BIAS - scores, article reviews, protocol conditions and more7. 7. Phillips5, Martin A. D 1 1. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. Examples: “Press carefully to engage,” “ Do not. SpectraMax® ABS. In. Background-subtracted data values (mean +/- SD) are graphed. . The BD plate dimensions are 5. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Images were visualized using a fluorescence microscope (Nikon, Japan) . Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. In vitro transcription (IVT) is an essential technique for RNA synthesis. Data are from ≥5 independent experiments. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). View specifications, prices, citations, reviews, and more. SpectraMax® Paradigm® Multi-Mode Detection Platform. 2. Images were taken using a fluorescence microscope (Nikon 80i, Japan). SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 1. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. Exhibit with us {{>currencyLabel}} Back. Background-subtracted data values (mean +/- SD) are graphed. Detection modalities are shown in Table 1-1. Background-subtracted data values (mean +/- SD) are graphed. StakMax Handling Stacker Base Unit. At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). 37. Five hours prior to when permeability will be measured, gavage mice with 0. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. SpectraMax Gemini XPS/EM Register or Sign In. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Dettmer4, Wayne A. Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 9000-0756. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. 2. overnight. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. 7°C and the bottom to 36. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SpectraMax® L (software version 5. Instruments Ltd. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. . Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. Large Molecule Bioanalysis. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. Table of Contents. RESEARCH PAPER Effect of particle size and surface charge of nanoparticles in penetration through intestinal mucus barrier Sony Priyanka Bandi & Yashada Sanjay Kumbhar & Venkata Vamsi Krishna VenugantiThe Fibrinogen standard curve was prepared as described in Section 10. Bioz Stars score: 98/100, based on 1 PubMed citations. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Mitochondrial respiration using high-resolution respirometry . Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. ATP standard curves read on SpectraMax microplate readers. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. No. SpectraMax® M4). In the M2, M5 and Plus 384, the light. Devices SpectraMax® M4). FlexStation ®, SpectraMax. STAKMAX. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Plot the fluorescence intensity versus the cell seeding number. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. ® reader to the computer. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 03 in x 3. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5 mg mL −1 with 0. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. 2. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Microplate Reader Shelf. Other technologies such as imaging. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. DMSO was used to solubilize the formazan crystals and absorbance was measured at 570 nm (Spectramax M4, Molecular devices, USA). N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. We have two NanoDrop micro-scale spectrophotometers, a Molecular Devices Spectramax M4 micro plate reader and a UVP gel documentation camera. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. 5. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. 313 reference gain check fail. The following document is intended to demonstrate setup of this instrument. 6. 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. /525 em. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. Establishment of mouse orthotopic xenograft bladder tumor model. The MDD was determined by calculating the mean of zero standard replicates (n=24) and. Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. Turn the instrument around so that the back of the instrument is. Western blot apparatus (BIO-RAD Laboratories). 4 kilograms). A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. WARNING! The instrument weighs approximately 36 pounds (16. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. 304 signal level saturation. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). Colony formation assays were performed to measure cell proliferation. normalized response (Graphpad Prism 8. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. Phillips5, Martin A. NCI-60 Human Tumor Cell Line Screen. Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 10. Results were expressed as a percentage relative to the control group (defined as 100%). . Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. Interpolated concentrations of native CRP in human serum and plasma samples. 3 or greater. After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). 0397 in x 3. ). Background-subtracted data values (mean +/- SD, n =2) are graphed. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). The drawing shows a standard microplate of 15 mm height covered with a lid. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. 0. 3, La Jolla, CA, USA). Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. com. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 9 pg/mL. . However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. After aerosol collection was completed, the impactor plates were rinsed with 0. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 1step assay with SpectraMax microplate readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). 3, La Jolla, CA, USA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 7. Cellular viability was assessed by using resazurin at 1. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. When a feature or capability applies to only certain readers, this exception is noted. l. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 3752 in as per SBS dimensions. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Table 2. 0 statistics software. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. , USA). 116 SpectraMax M5 and M5e Multi-Mode. 4) and cells were stained with 150 nM TSQ for 1 min. . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 2. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The aim of the present study was to investigate the preventive effects of. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. SpectraMax® Paradigm® Multi-Mode Detection Platform. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. D 47 7. *Statistical significance: p < 0. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 2 only) SpectraMax M-series Microplate readers. (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Moving a SpectraMax Multi-Mode Microplate Reader. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Measurement of Mitochondrial Membrane Potential by JC-1. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. instruments you must first insert an adapter plate. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Results were normalized and expressed as a percentage of live cells. 2. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 4. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. The Multi-mode readers are top and bottom read. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). 3. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. • Tel. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Parameter SpectraMax iD5 SpectraMax i3x. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. SpectraMax Microplate Readers. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. . SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. 2 only). 7°C (gradient heating design). Data are presented as mean values ± standard. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. . (Shanghai, China). SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. 1. 39. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. The following document is intended to demonstrate setup of this instrument. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 1. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 1 & 7. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. 5 pM 2. trophotometer (SpectraMax M4, Molecular Devices, USA). To report an issue with this product, Brief content visible, double tap to read full content. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). reader (SpectraMax M4, Molecular Devices; Sunnyvale, CA, USA) at 540 nm absorbance. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. 161 likes. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]. Immunodetection Protocols for Cell Culture.